PCR Water

PCR Water

PCR and Nuclease-Free Water is a molecular biology-grade product suitable for sample dilution and use as an ultra-pure solvent for PCR reactions and other molecular biology reactions. PCR Water is free from DNase, RNase, protease, and DNA, as verified by our strict quality control methods. Meanwhile, Nuclease-Free Water is guaranteed to be nuclease-free, but not tested for contaminating nucleic acids. Ensure maximum reassurance and reduce variability in your molecular experiments by using PCR Water in all workflows and with sensitive samples. Features: Free from DNase, RNase & protease No DNA, ensuring no background contamination (PCR Water) Strictly quality controlled, ensuring maximum reassurance
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CAT No.ProductSizePrice
PB40.40-05PCR Water5 mL (1 x 5 mL)Contact us
PB40.40-06PCR Water5 mL (5 x 1 mL)Contact us
PB40.40-50PCR Water50 mL (1 x 50 mL)Contact us
PB40.40-51PCR Water50 mL (50 x 1 mL)Contact us

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Additional Information

PCR Water can be used as a reaction component with all PCR Biosystems reagents and all other manufacturers' products where the corresponding instructions or documentation call for the use of PCR grade or ultra-pure water.

Furthermore, this product undergoes rigorous testing to ensure maximum reassurance when setting up molecular biology reactions. The activity of DNase, RNase, and protease, as well as DNA, are all below the detection threshold of our stringent assays.

Product packaging sizes are available in small (5 mL) and large (50 mL) volumes, and in both aliquoted form (5 x 1 mL and 50 x 1 mL) or single bottle (1 x 5 mL and 1 x 50 mL), maximizing convenience based on your workflow and throughput requirements.

Nuclease-free water is provided as a more economical alternative that can be used for working with proteins, or other molecular applications where contaminating nucleic acids are not a concern. This product can be used in PCR reactions. However, there is a small possibility that the reactions may be impacted by trace DNA.

Why should you use PCR Water in your molecular biology workflow?

Using ultra-pure deionized water in PCR workflows offers several significant benefits, primarily eliminating the risk of contamination that can adversely affect the sensitivity and accuracy of PCR reactions. In PCR, even trace levels of impurities such as ions, organic compounds, or microbial contamination can lead to non-specific amplification or inhibition of polymerase enzymes, thereby minimizing the accuracy and reliability of results. The ultra-pure deionized water has undergone rigorous purification processes to remove these impurities, ensuring high consistency and reproducibility in PCR assays. It provides a stable and controlled environment, enhancing the efficiency of DNA polymerase, facilitating accurate amplification of target sequences. This level of purity is particularly critical in applications requiring high sensitivity, such as quantitative PCR (qPCR) and diagnostic tests, where the integrity of the results directly impacts research outcomes and patient care.

Applications

  • PCR
  • qPCR
  • All Molecular Biology Reactions
  • Sample Dilution
  • Use as a Negative Control

Technical Specifications

PCR Water

Component 5 mL 5 mL 50 mL 50 mL
PCR Water 1 x 5 mL 5 x 1 mL 1 x 50 mL 50 x 1 mL

Nuclease-Free Water

Component 100 mL 500 mL
Nuclease-Free Water 1 x 100 mL 1 x 500 mL

PCR Water

Component 5 mL 5 mL 50 mL 50 mL
PCR Water 1 x 5 mL 5 x 1 mL 1 x 50 mL 50 x 1 mL

Nuclease-Free Water

Component 100 mL 500 mL
Nuclease-Free Water 1 x 100 mL 1 x 500 mL

Reaction Information

Reaction Volume Storage
Not applicable Upon receipt, the product can be stored at room temperature. Once opened, care should be taken to avoid contamination and storage at -30 to -20 °C is recommended. The product can be stored indefinitely before first use.

Frequently Asked Questions (FAQs)

Yes. In fact, using PCR Water when preparing and diluting molecular biology samples is highly recommended. This minimizes the risk of contamination for the samples themselves and also ensures that ion content is minimized, thereby reducing potential interference with enzymatic reactions.

PCR Water from PCR Biosystems can be used with all common molecular biology derivatives, regardless of manufacturer or type of application, where the respective manuals or instructions call for molecular biology, PCR-grade, or ultra-pure water.

This means that the activity of DNase, RNase, and protease is below the detection limits of our stringent quality control assays. The product is guaranteed free from these enzymatic activities, by this definition, prior to first use. Once opened, care should be taken to avoid contamination, especially if the same aliquot will be stored after first opening.
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