IsoFast® Hot Start Bst Polymerase & Mixes

IsoFast® Hot Start Bst Polymerase & Mix represents the latest development and improvement in isothermal amplification technology, designed to deliver unmatched specificity and speed in DNA amplification processes and to meet the requirements in molecular diagnostics and research. Whether you are working with Loop-mediated Isothermal Amplification (LAMP) or other isothermal amplification methods, IsoFast® Hot Start Bst Polymerase 2x Mix ensures excellent sensitivity and reliability. Our reagents leverage the power of hot-start Bst DNA polymerase enzyme, ensuring minimal background amplification and maximizing target specificity. With IsoFast® Hot Start, your isothermal amplification experiments will be faster, more efficient, and easier than ever, setting new standards for molecular biology techniques in both diagnostic applications and research efforts.

Hot start is crucial in isothermal amplification techniques because it helps prevent non-specific amplification and false positive results. Isothermal amplification methods, such as LAMP, rely on specific primers and enzymes to amplify a target sequence at a constant temperature, typically between 60°C and 65°C. These techniques are widely used in various applications, including molecular diagnostics and pathogen detection.

Why is hot start important in isothermal amplification?

Preventing non-specific amplification in isothermal Bst polymerase reactions:

In isothermal amplification, it is important to ensure that the amplification process targets only the specific sequence you are interested in. Non-specific amplification can occur when primers bind to unintended regions of the sample or when amplification enzymes initiate too early. Hot start techniques involve inhibiting the amplification reaction until the reaction reaches the desired temperature. This prevents the formation of non-specific products in the early stages of the reaction when the temperature is still below the optimal amplification temperature.

Enhancing specificity of isothermal amplification by Bst polymerase:

By blocking early amplification, hot start methods increase the specificity of the isothermal amplification reaction. This means the reaction is less likely to amplify non-target sequences, reducing the risk of false positive results.

Improving sensitivity in isothermal amplification with Bst polymerase:

Hot start techniques can also improve the sensitivity of isothermal amplification assays. By minimizing non-specific amplification, more reagents are available for amplifying the target sequence, enhancing the efficiency of the reaction and the limit of detection.